Anti-CD Antibodies 제품을 소개합니다.

CD differentiation antigen은 백혈구 및 면역 체계와 관련된 기타 세포에서 발현되는 세포 표면 분자입니다. 이러한 CD 마커는 flow cytometry를 이용하여 특정 세포 집단을 식별하고 분리하는 데 일반적으로 사용됩니다. Anti-CD antibodies는 표적 CD 항원을 특이적으로 인식하며 세포 활성화, 하위 집단 식별, 검출 및 세포 분류에 널리 적용됩니다. GenScript는 세포 치료 프로젝트의 개발 및 제조를 지원하기 위해 널리 사용되는 CD 마커를 표적으로 하는 다양한 Anti-CD antibodies 제품군을 제공합니다.

[Product Highlights]

[Product Performance]

• High Consistency

Figure 1. Lot-to-lot consistency was tested using four batches of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) on Jurkat/IL-2-Luc Promoter cells. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway. Data showed that GenScript Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) has a high lot-to-lot consistency.

Figure 2. Lot-to-lot consistency was tested using four batches of Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) on Jurkat/IL-2-Luc Promoter cells. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway. Data showed that GenScript Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) has a high lot-to-lot consistency.

• High Stability

Figure 3. Stability of GenScript GMP Anti-Human CD8 Antibody (F002), mAb, Mouse (Cat. No. GMPA02212) was tested. The data of long-term stability, accelerated stability, and freeze-thaw cycle stability indicated that this GMP antibody is very stable under various storage conditions.

[Application Data]

• T Cell Activation Using Anti-Human CD3 and Anti-Human CD28 Antibodies

Anti-CD3 및 Anti-CD28 항체는 T 세포 표면에 발현되는 해당 CD 마커에 결합하여 각각 T 세포 활성화를 위한 1차 및 2차 신호를 제공합니다. 이러한 공동 자극 활성화 신호는 빠르고 강력한 T 세포 증식을 유도하며, 이는 적응 면역 반응 및 면역 치료에 필수적인 역할을 합니다.

• T cell Activation Performance using Anti-Human CD3 and Anti-Human CD28 Antibodies

Figure 1. GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) was used for primary T cell activation together with anti-Human CD28 antibody. The percentage of activated T cells were measured by flow cytometry. Data showed that GenScript GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse outperformed the competing products in activating primary T cells.

• Use of Anti-Human CD3 and Anti-Human CD28 Antibodies for Studying Signal Response in Reporter Cell Lines

Figure 2. CHO-K1/CD80 cells were plated 15 h before adding Jurkat/IL-2-Luc Promoter cells, then Jurkat/IL-2-Luc Promoter cells were stimulated with a serial of concentrations of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) for 5 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

Figure 3. Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) and 1 μg/mL of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) for 5 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

• Primary T cell Characterization and Cytokine Secretion Detection after Cell Activation

Figure 4. Primary T cells were stimulated with 5 μg/mL of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) (left), competitor’s anti-Human CD3 antibody (middle), negative control (NC, right) as well as anti-Human CD28 antibody (all panels) for 72 h, respectively. CD25/CD69 expression was detected by flow cytometry to characterize cell activation.

Figure 5. Primary T cell clumping was observed by a microscope after T cell activation (72 h) (right) using Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) and Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213). The inactivated T cells were presented on the left image.

Figure 6. Interferon γ (IFN-γ) secretion by activated primary T cells using Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) and anti-Human CD28 antibody. Data showed that GenScript anti-Human CD3 antibody outperformed competing product.

Figure 7. Interferon γ (IFN-γ) secretion by activated primary T cells using Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) and anti-Human CD3 antibody. Data showed that GenScript anti-Human CD28 antibody outperformed competing product.

• PBMC Staining for T Cell Population Identification Using Anti-Human CD4 and Anti-Human CD8 Antibodies

Anti-CD antibodies는 세포에 발현되는 CD 마커를 특이적으로 인식하여 세포를 다양한 세포 유형 또는 기능적 세포 하위 집단으로 분류하는 데 사용할 수 있습니다. 예를 들어, CD4와 CD8은 말초혈액 단핵세포(PBMC) 염색 분석에서 CD4+ 보조 T 세포와 CD8+ 세포독성 T 세포를 식별하는 데 가장 일반적으로 사용되는 마커입니다.

Figure 8. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Human CD4 Antibody (F001), mAb, Mouse (Cat. No. A02211) (left), competitor’s anti-human CD4 antibody (middle), mouse IgG1 negative control (NC, right) as well as with anti-Human CD3 [BV510] antibody (all panels). Flow cytometry was performed for further analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD.

Figure 9. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Human CD8 Antibody (F002), mAb, Mouse (Cat. No. A02122) (left), competitor’s anti-Human CD8 antibody (middle), mouse IgG1 negative control (right) as well as with anti-Human CD3 [BV510] antibody (all panels). Flow cytometry was performed for further analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD.