Human Methylated & Non-methylated DNA Set

Human Methylated & Non-methylated DNA Set는 두 가지 DNA control (CpG-methylated human DNA standard, non-methylated human DNA standard)과 EZ DNA Methylation 제품군과 함께 사용할 수 있도록 특별히 설계된 primer 세트로 구성됩니다. 이 DNA 세트는 DNA의 bisulfite conversion 효율을 평가하기 위한 양성 및 음성 대조군으로 사용할 수 있습니다. non-methylated human DNA는 DNA methyltransferase인 DNMT1(-/-)과 DNMT3b(-/-)가 모두 결핍된 HCT116 DKO 세포주에서 정제되었습니다. methylated DNA standard는 CpG 부위에서 효소적으로 메틸화된 정제된 HCT116 DKO DNA입니다.

Highlights

Technical Specifications

Ideal Controls for BSP and MSP Analysis

Example MSP experiment using MSP designed primers for RASSF1. Sample 1 is positive for a Methylated Template. Sample 2 is positive for a NonMethylated Template and Sample 3 contains Methylated and Non-Methylated Templates. MSP experiment also shows proper controls: Meth (+) DNA Control D5014-2 Human Methylated DNA, Meth (-) DNA Control D5014-1 Human Nonmethylated DNA. 2% Agarose Gel, 130V for 35 mins. M = Methylated specific primers, U = Non-Methylated specific primers.

DNA Methylation Human DNA Standards